Laboratory diagnosis of emerging human coronavirus infections - the state of the art.
Identifieur interne : 000519 ( Main/Exploration ); précédent : 000518; suivant : 000520Laboratory diagnosis of emerging human coronavirus infections - the state of the art.
Auteurs : Michael J. Loeffelholz [États-Unis] ; Yi-Wei Tang [République populaire de Chine]Source :
- Emerging microbes & infections [ 2222-1751 ] ; 2020.
Descripteurs français
- KwdFr :
- Analyse sur le lieu d'intervention, Antigènes viraux (analyse), Flambées de maladies, Humains, Infections à coronavirus (diagnostic), Infections à coronavirus (mortalité), Infections à coronavirus (épidémiologie), Maladies transmissibles émergentes (diagnostic), Maladies transmissibles émergentes (mortalité), Maladies transmissibles émergentes (épidémiologie), Pandémies, Pneumopathie virale (diagnostic), Pneumopathie virale (mortalité), Pneumopathie virale (épidémiologie), Prélèvement biologique, RT-PCR, Réaction de polymérisation en chaine en temps réel, Syndrome respiratoire aigu sévère (diagnostic), Syndrome respiratoire aigu sévère (mortalité), Syndrome respiratoire aigu sévère (épidémiologie), Techniques de laboratoire clinique, Tests sérologiques.
- MESH :
- analyse : Antigènes viraux.
- diagnostic : Infections à coronavirus, Maladies transmissibles émergentes, Pneumopathie virale, Syndrome respiratoire aigu sévère.
- mortalité : Infections à coronavirus, Maladies transmissibles émergentes, Pneumopathie virale, Syndrome respiratoire aigu sévère.
- épidémiologie : Infections à coronavirus, Maladies transmissibles émergentes, Pneumopathie virale, Syndrome respiratoire aigu sévère.
- Analyse sur le lieu d'intervention, Flambées de maladies, Humains, Pandémies, Prélèvement biologique, RT-PCR, Réaction de polymérisation en chaine en temps réel, Techniques de laboratoire clinique, Tests sérologiques.
English descriptors
- KwdEn :
- Antigens, Viral (analysis), Betacoronavirus (genetics), Betacoronavirus (immunology), Clinical Laboratory Techniques, Communicable Diseases, Emerging (diagnosis), Communicable Diseases, Emerging (epidemiology), Communicable Diseases, Emerging (mortality), Coronavirus Infections (diagnosis), Coronavirus Infections (epidemiology), Coronavirus Infections (mortality), Disease Outbreaks, Humans, Pandemics, Pneumonia, Viral (diagnosis), Pneumonia, Viral (epidemiology), Pneumonia, Viral (mortality), Point-of-Care Testing, Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction, Serologic Tests, Severe Acute Respiratory Syndrome (diagnosis), Severe Acute Respiratory Syndrome (epidemiology), Severe Acute Respiratory Syndrome (mortality), Specimen Handling.
- MESH :
- chemical , analysis : Antigens, Viral.
- diagnosis : Communicable Diseases, Emerging, Coronavirus Infections, Pneumonia, Viral, Severe Acute Respiratory Syndrome.
- epidemiology : Communicable Diseases, Emerging, Coronavirus Infections, Pneumonia, Viral, Severe Acute Respiratory Syndrome.
- genetics : Betacoronavirus.
- immunology : Betacoronavirus.
- mortality : Communicable Diseases, Emerging, Coronavirus Infections, Pneumonia, Viral, Severe Acute Respiratory Syndrome.
- Clinical Laboratory Techniques, Disease Outbreaks, Humans, Pandemics, Point-of-Care Testing, Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction, Serologic Tests, Specimen Handling.
Abstract
The three unprecedented outbreaks of emerging human coronavirus (HCoV) infections at the beginning of the twenty-first century have highlighted the necessity for readily available, accurate and fast diagnostic testing methods. The laboratory diagnostic methods for human coronavirus infections have evolved substantially, with the development of novel assays as well as the availability of updated tests for emerging ones. Newer laboratory methods are fast, highly sensitive and specific, and are gradually replacing the conventional gold standards. This presentation reviews the current laboratory methods available for testing coronaviruses by focusing on the coronavirus disease 2019 (COVID-19) outbreak going on in Wuhan. Viral pneumonias typically do not result in the production of purulent sputum. Thus, a nasopharyngeal swab is usually the collection method used to obtain a specimen for testing. Nasopharyngeal specimens may miss some infections; a deeper specimen may need to be obtained by bronchoscopy. Alternatively, repeated testing can be used because over time, the likelihood of the SARS-CoV-2 being present in the nasopharynx increases. Several integrated, random-access, point-of-care molecular devices are currently under development for fast and accurate diagnosis of SARS-CoV-2 infections. These assays are simple, fast and safe and can be used in the local hospitals and clinics bearing the burden of identifying and treating patients.
DOI: 10.1080/22221751.2020.1745095
PubMed: 32196430
Affiliations:
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Le document en format XML
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the beginning of the twenty-first century have highlighted the necessity for readily available, accurate and fast diagnostic testing methods. The laboratory diagnostic methods for human coronavirus infections have evolved substantially, with the development of novel assays as well as the availability of updated tests for emerging ones. Newer laboratory methods are fast, highly sensitive and specific, and are gradually replacing the conventional gold standards. This presentation reviews the current laboratory methods available for testing coronaviruses by focusing on the coronavirus disease 2019 (COVID-19) outbreak going on in Wuhan. Viral pneumonias typically do not result in the production of purulent sputum. Thus, a nasopharyngeal swab is usually the collection method used to obtain a specimen for testing. Nasopharyngeal specimens may miss some infections; a deeper specimen may need to be obtained by bronchoscopy. Alternatively, repeated testing can be used because over time, the likelihood of the SARS-CoV-2 being present in the nasopharynx increases. Several integrated, random-access, point-of-care molecular devices are currently under development for fast and accurate diagnosis of SARS-CoV-2 infections. These assays are simple, fast and safe and can be used in the local hospitals and clinics bearing the burden of identifying and treating patients.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li><li>États-Unis</li></country><region><li>Californie</li></region></list><tree><country name="États-Unis"><region name="Californie"><name sortKey="Loeffelholz, Michael J" sort="Loeffelholz, Michael J" uniqKey="Loeffelholz M" first="Michael J" last="Loeffelholz">Michael J. Loeffelholz</name></region></country><country name="République populaire de Chine"><noRegion><name sortKey="Tang, Yi Wei" sort="Tang, Yi Wei" uniqKey="Tang Y" first="Yi-Wei" last="Tang">Yi-Wei Tang</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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